Postdoctoral position in virology
Reference
1497254957
Deadline for applications
07/07/2017
Date of publication
06/12/2017

Details

Details on the type of contract
Postdoctoral contract
Duration of contract
2 years
Beginning
09/01/2017

Remuneration
2338.33 euros brut/mois (selon expérience)

Assignment

Name of unit of assignment
UMR1161 VIRO Virologie
Address of unit of assignment
ENVA - Maisons Alfort 7 avenue du Général de Gaulle - 94704 MAISONS-ALFORT CEDEX
Region of assignment
Paris Region

Description

Working environment

VOTRE MISSION ET VOS ACTIVITÉS

A postdoctoral position is available at the UMR 1161 Virology, a joined research unit between INRA (French National Institute for Agricultural Research), ANSES (French Agency for Food, Environmental and Occupational Health & Safety) and ENVA (National veterinary school of Alfort) that is located at the veterinary school at Maisons-Alfort. This unit is composed of 5 research groups: i-Adenoviral vectors and vaccines, ii-Emerging viruses of ruminants, iii-Enteric viruses and species barrier, iv-Biology of Picornaviruses and v-Neurovirology of zoonosis. Several national reference laboratories as well as the European reference laboratory (EU-RL) for equine diseases are also present within the UMR.

A position is available within the « Enteric Viruses and Species Barrier » team that studies two viral families causing emerging zoonosis: hepatitis E viruses (HEV) and coronaviruses (CoV). The group investigates particularly the reservoirs (domestic and wild fauna) and the routes of transmission (foodborne and environmental) of these zoonotic viruses. An important objective is also to identify and characterise the virulence factors involved in species barrier crossing. The team is funded by projects from the National Agency for Research (ANR) (EPICOREM and HEVISTAR), the National Agency for AIDS and viral hepatitis Research (ANRS) and European projects (PREDEMICS and CoVetLab projects).

 

Your will be hired as a post-doc within the national grant HEVISTAR that aims to identify and characterise proteins encoded by interferon-stimulated genes (ISG) with antiviral activity against HEV that are involved in the inter-species transmission of HEV. This will provide a better understanding of host and viral factors contributing to HEV adaptation and species barrier crossing.

You will be in charge of the design, development and implementation of experimental systems required to carry out this project. This involves using customised PCR arrays to detect the expression of hundreds of human and swine ISGs in human hepatocytes infectedin vitrowith HEV and in the liver of pigs experimentally infected with HEV. You will use a medium-throughput approach to screen hundreds of human ISGs to identify ISG-encoded proteins with antiviral activities against HEV infection in collaboration with Dr. Sam Wilson from the MRC-University of Glasgow Centre for Virus Research. You will also be involved in the characterisation of the mechanisms of action of the ISGs of interest. 

 

You will use molecular and cellular biology techniques such as nucleic acid extraction, qRT-PCR, PCR arrays, cell culture, viral infections, immunofluorescence, HEV replicon system and western-blotting. You will be involved in the design of animal experimentations and in the processing of samples and their analysis.

 

 

 You will be particularly in charge of:

-       Designing, optimising and implementing the techniques required for the project (PCR arrays, medium-throughput ISG screen, HEV replicon system).

-       Processing and analysing samples from animal experiments.

-       Scientific and technological monitoring.

-       Data analysis (result analysis, interpretation and validation) using appropriate software.

-       Promoting and communicating the results of this project in English by writing scientific papers and presenting oral communications.

Training and skills required

LE PROFIL QUE NOUS RECHERCHONS : 

Formation recommandée: PhD in biological sciences.

Connaissances souhaitées: Knowledge in cellular biology, virology, biochemistry, molecular biology and laboratory biosafety regulations.

Expérience appréciée: Expertise in cellular biology, molecular biology, virology and dedicated software.

Aptitudes recherchées: Motivation, rigour, excellent communication skills to interact with multiple persons, planning skills, ability to adapt easily to new environment and to act independently and take initiatives.

Contact

Name
Virginie Doceul
Email
virginie.doceul@vet-alfort.fr