Young post-doctoral researcher in extracellular vesicles biology
Reference
1490703537
Deadline for applications
05/01/2017
Date of publication
03/28/2017

Details

Details on the type of contract
Temporary assignment
Duration of contract
18 months
Beginning
06/01/2017

Assignment

Name of unit of assignment
UMR1253 STLO Science et Technologie du Lait et de l'Oeuf
Address of unit of assignment
INRA-Agrocampus Rennes - Rte St Brieuc 65 rue de Saint-Brieuc - 35042 RENNES CEDEX
Region of assignment
Brittany

Description

Working environment

Context and job description: Food fermentation by bacteria is one of the oldest and sustainable forms of biopreservation practice. In addition to improving organoleptic characteristics and nutritional value of raw
materials, it has been accepted that fermented products, in particular dairy products, contribute in improving human health. Despite numerous studies deciphering health attributes of dairy bacteria including probiotics, knowledge about mechanisms underlying their beneficial effects remains limited.

Cells in all domain of life release extracellular vesicles (EVs) into the environment. EVs serve as intercellular mediators of communication in carrying regulatory molecules from donor cells to recipient cells. We hypothesis that EVs can be a molecular link between the beneficial properties of dairy bacteria and their functionalities related to human health. A better knowledge of mechanisms involved in bacterial cell to host cell interactions will allow the design of new fermented milk products with improved health properties. EVs research is a quickly developing and attractive field with great potential. However, there is little EV-related research with dairy Gram-positive bacteria.

The challenge of the postdoc’s work will be to demonstrate the involvement of EVs as mediators of beneficial properties of dairy bacteria. In particular, He/She will characterize EVs cargo (proteins, nucleic acids, metabolites) from a relevant set of bacteria (e.g. Lactococcus lactis, Streptococcus thermophilus, Lactobacillus species, Propionibacterium freudenreichii) and elucidate their impacts on food ecosystem functioning, microbiota homeostasis and host health. Recently, we showed that EVs derived from P. freudenreichii, a dairy probiotic, contain anti-inflammatory proteins suggesting that EVs function as immunomodulatory molecules. Environmental and genetic factors modulating EVs production and cargo will also be addressed in a model organism of dairy bacteria.

 

Hosting conditions and research facilities: Young researcher will be welcomed at the STLO INRA laboratory(140 persons) in Rennes. The research group where He/She will work is composed of 25 permanent staff (scientists, engineers and technicians) and 5 to 10 students (master, PhD). The lab hosts a dairy platform where all kind of dairy products can be manufactured in highly controlled conditions. It is also equipped with mass spectrometry (Orbitrap), dynamic digestion simulator, AFM, confocal microscopy and has a full access to anelectronic microscopy platform.

The group has well-known expertise in molecular microbiology, milk biochemistry, proteome and volatilome, transcriptome, microscopy and in genomic (diversity, function) of diary bacteria.

Training and skills required

Candidate profile and skills: Young international researchers who reside abroad (or have arrived in France recently) and young French researchers who have carried out research outside France for at least 12 months in the last 3 years are eligible. Applicants must have obtained their PhD in a relevant field after 2013. Skills in EV biology and cellular biology will be greatly appreciated. We are looking for a highly motivated and autonomous researcher interested in microbiology, molecular biology, genomics and cell-cell communication. Scientific curiosity, professional conscience, ability for team-work, good skills in publication writing are of foremost importance.

 

Mode of application: To apply, please send an email to Eric Guédon (eric.guedon@inra.fr) containing a shortletter of motivation, a detailed CV (including a brief description of research interests, previous employments, and publication list), and contact details of at least two references. Deadline of submission: 1st May of 2017.

Contact

Name
Eric Guédon
Email
eric.guedon@inra.fr